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KMID : 0043319990220010013
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Archives of Pharmacal Research
1999 Volume.22 No. 1 p.13 ~ p.17
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The Kinetic Characteristics of K228G Mutant Horse Liver Alcohol Dehydrogenase
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Cho Sun-Hyoung
Ryu Ji-Won
Lee Kang-Man
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Abstract
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The kinetic constants and the reaction mechanism of the K228G mutant horse liver alcohol dehyrogenase isoenzyme E (HLADH-E) were compared to the wild-type enzyme. All the Km and Ki constants of the mutant enzyme for NAD+, ethanol, acetaldehyde and NADH were larger than those of the wild-type enzyme. The dissociation constants for the NADH and (Kiq and Kia) were greatly increased by 130-and 460-fold, respectively. The product inhibition patterns suggested that the reaction mechanism of the mutant enzyme was changed to Random Bi Bi. These results could attribute to the increase in the dissociation rate of coenzyme with the substitution at Lys-228 residue.
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KEYWORD
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K228G, Horse liver alcohol dehydrogenase, Kinetic constants, Random Bi Bi
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